Modifying Enzymes
Worthington Biochemical Corporation HEPATOCYTE ISOLATION
A component of the Hepatocyte and Papain Dissociation Systems. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease.
Discovery Life Sciences HUMAN UGT2B15 0.5ML@5MG/ML
BD Gentest, Human UGT2B15, Regulatory Status is RUO, 0.5mL@5mg/mL
Discovery Life Sciences UGT CONTROL 0.5ML@5MG/ML
BD Gentest, UGT Control, Regulatory Status is RUO, 0.5mL@5mg/mL
New England Biolabs, Inc. yDcpS – 4000 units
yDcpS is the scavenging decapping enzyme from S. cerevisiae that hydrolyzes the phosphodiester bond between the gamma and beta phosphates of m7G-capped mRNA, producing diphosphorylated 5 ends and m7GMP. yDcpS is capable of decapping mRNAs of various lengths, decaps Cap0 and Cap1 with equal efficiency, and the diphosphorylated 5 end it produces is suitable for recapping with Vaccinia Capping Enzyme.
Roche Diagnostics PROTEASE 1
Protease 1, Component: Proprietary ingredients, 45 to 55%, Sodium azide, CAS No.: 26628-22-8, 0.1%, Appearance: Clear, Colorless liquid, Odorless, Solubility: Miscible in water, pH: neutral, Stability: stable
STEMCELL Technologies Hyaluronidase, Size: 50000 U
Hyaluronidase is a polysaccharidase consisting of a single polypeptide chain of 450 amino acid residues and contains four disulfide bonds. Hyaluronidase is glycosylated and contains 5% mannose and 2.17% glucosamine (Borders & Raftery). Hyaluronidase cleaves endo-N-acetlyhexosaminic bonds in hyaluronic acid and chondroitin sulfate A and C to tetrasaccharide residues, as hyaluronic acid and chondroitin sulfate are often found in connective tissues. Hyaluronidase is frequently used with other proteases such as collagenase.
Sigma Aldrich Fine Chemicals Biosciences beta(1->4)-Galactosidase, positionally specific from Streptococcus pneumoniae recombinant, expressed in E. coli, buffered aqueous solution | 9031-11-2 | MFCD01092684 | 1VL
beta(1->4)-Galactosidase, positionally specific from Streptococcus pneumoniae recombinant, expressed in E. coli, buffered aqueous solution | 9031-11-2 | MFCD01092684 | 1VL
Sigma Aldrich Fine Chemicals Biosciences beta-(1->3,4,6)-Galactosidase, positionally specific recombinant, expressed in E. coli, buffered aqueous solution | 9031-11-2 | MFCD01092682 | 1VL
beta-(1->3,4,6)-Galactosidase, positionally specific recombinant, expressed in E. coli, buffered aqueous solution | 9031-11-2 | MFCD01092682 | 1VL
Ubpbio HUMAN UBE1, 25UG
E1 Activating Enzymes – UbE1 (UBPBio catalog# B1100, B1101) – Purified from sf9 insect cells. UBE1 is an E1 enzyme within the E1, E2, E3 cascade which conjugates Ub to protein substrates. UBE1 activates Ub by catalyzing an ATP – dependent reaction; Ub is conjugated onto the catalytic Cys residue of UBE1 by formation of a thioester bond. The activated Ub is then passed onto a Ub-conjugating enzyme E2; Ub charged E2 binds a Ub ligase E3 that directly binds substrate proteins. Ub is transferred to the HECT domain E3s prior to being conjugated on substrate proteins. For RING domain E3s, Ub is transferred directly from an E2 to substrate proteins.
Worthington Biochemical Corporation Deoxyribonuclease I, Ribonuclease&Protease Free, 2500un
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Lyophilized in vial containing glycine and calcium as stabilizer. Store at 2-8°C. Protect from moisture. Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Sigma Aldrich Fine Chemicals Biosciences Lipase from Candida rugosa Type VII, ≥700 unit/mg solid | 9001-62-1 | MFCD00131509 | 100g
Lipase from Candida rugosa Type VII, ≥700 unit/mg solid | 9001-62-1 | MFCD00131509 | 100g
Sigma Aldrich Fine Chemicals Biosciences Cellulase from Trichoderma reesei aqueous solution, ≥700 units/g | 9012-54-8 | MFCD00081510 | 50mg
Cellulase from Trichoderma reesei aqueous solution, ≥700 units/g | 9012-54-8 | MFCD00081510 | 50mg